Additional Selective and Differential Cell Culture Media
NEOGEN m-FC Agar, 10 kg
m FC Agar is used with rosolic acid for the detection and enumeration of fecal coliforms by membrane filtration in a laboratory setting. m-FC Agar is not intended for use in the diagnosis of disease or other conditions in humans. Geldreich et al. formulated a medium to enumerate fecal coliforms (FC) using the membrane filter (m) technique without prior enrichment. Many standard method membrane filtration procedures recommend m-FC media for testing water. The American Public Health Association (APHA) specified m-FC media and incubation at 44.5 ± 0.5°C in the fecal coliform procedure and other tests. The Association of Official Analytical Chemists (AOAC) specifies m-FC Agar for detecting total coliforms and fecal coliforms in foods. The US Environmental Protection Agency specified using m-FC media in fecal coliform methods for testing water by the direct MF method or the delayed-incubation MF methods.For more information visit https://www.neogen.com/solutions/Microbiology/m-fc-agar
NEOGEN Phenylethanol Agar, 500 g
Phenylethanol Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Phenylethanol Agar is not intended for use in the diagnosis of disease or other conditions in humans. Brewer and Lilley reported the addition of phenylethanol to a nutritive medium permitted growth of Gram-positive organisms, but markedly to completely inhibited growth of Gram-negative organisms. Phenylethanol Agar inhibits swarming of Proteus spp., and can be used to selectively isolate anaerobic bacteria from specimens with mixed flora. Phenylethanol Agar is specified for use in several reference methods.For more information visit https://www.neogen.com/solutions/Microbiology/phenylethanol-agar
NEOGEN Buffered Sodium Chloride - Peptone Broth pH 7.0, 500 g
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans.Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.For more information visit https://www.neogen.com/solutions/Microbiology/buffered-sodium-chloride-peptone-broth-ph-7
NEOGEN Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest Casein, 500 g
TSB, Modified with 20mg Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. TSB Modified w/20 mg Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. TSB, Modified w/20 mg Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing. This formulation conforms to the USDA/FSIS Microbiology Laboratory Guidebook.For more information visit https://www.neogen.com/solutions/Microbiology/tryptic-soy-broth-modified-with-20-mg-novobiocin-and-acid-digest-casein
NEOGEN LB Agar, Miller, 500 g
LB Agar (Miller), a nutritionally rich medium designed for growth of pure cultures of recombinant strains, is based on the formulation described by Miller. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to a high copy number and do not require selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol may be added to inhibit host synthesis and prevent replication of the bacterial chromosome.LB Agar, Miller contains 10 g/L of sodium chloride, different from the levels in Lennox formulations. This allows the researcher to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose or antibiotics.For more information visit https://www.neogen.com/solutions/Microbiology/lb-agar-miller
NEOGEN HC Agar Base, 500 g
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.Methods for isolating molds from cosmetic products require incubation for 5 - 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.For more information visit https://www.neogen.com/solutions/Microbiology/hc-agar-base
NEOGEN Columbia Broth, 500 g
Columbia Broth is used for the cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Columbia Broth is not intended for use in the diagnosis of disease or other conditions in humans.Columbia Broth is prepared according to the formula described by Morello and Ellner. During their study, Columbia Broth was developed as a medium for blood cultures and found superior to a commonly used broth for growth of Staphylococcus aureus, E. coli, and streptococci.For more information visit https://www.neogen.com/solutions/Microbiology/columbia-broth
NEOGEN Sorbitol MacConkey Agar, 500 g
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans. Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases. MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.For more information visit https://www.neogen.com/solutions/Microbiology/sorbitol-macconkey-agar
NEOGEN Universal Beer Agar, 10 kg
Universal Beer Agar is used for the cultivation of bacteria and yeasts encountered in the brewing industry in a laboratory setting. Universal Beer Agar is not intended for use in the diagnosis of disease or other conditions in humans. Universal Beer Agar was developed by Kozulis and Page and used as a basal medium supplemented with beer. The developers compared this formula with other media commonly used in breweries for detecting microbial contamination. The characteristics of Universal Beer Agar are closer to natural environmental conditions found in a typical brewery. Universal Beer Agar supports the growth of more lactic acid bacteria and yields larger colonies in a shorter time than traditional brewer’s media. Supplementing this medium with beer has produced a selective environment for organisms that are adapted to existent conditions in the brewery.For more information visit https://www.neogen.com/solutions/Microbiology/universal-beer-agar
NEOGEN Urea Agar Base (Christensen), 500 g
Urea Agar Base is used with agar for the differentiation of microorganisms on the basis of urease production in a laboratory setting. Urea Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Christensen devised a urea agar medium containing peptone and dextrose that had a reduced buffer content. The medium supported the vigorous growth of many Gram-negative, enteric bacilli and readily permitted observation of urease production. Urea Agar Base may be used as a screening medium (along with Triple Sugar Iron Agar) for the selection of Salmonella and Shigella cultures for serologic classification. Urea Agar Base is also used to detect production of urease by yeast. Urease production is an important differential test in microbiology and outlined in standard methods.For more information visit https://www.neogen.com/solutions/Microbiology/urea-agar-base-christensen
NEOGEN Yeast Extract Dextrose Chloramphenicol Agar, 5 kg
Yeast Extract Dextrose Chloramphenicol Agar is a selective medium for the enumeration of yeasts and molds in milk and other dairy products, and is not intended for use in the diagnosis of disease or other conditions in humans. The medium is said to have superior storage properties to O.G.Y.E. and also has the advantage of incorporating an autoclavable supplement.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract-dextrose-chloramphenicol-agar
NEOGEN LEE Broth, 500 g
Listeria Express Enrichment Broth (LEE Broth) is a selective enrichment broth for the detection of Listeria from foods and is not intended for use in the diagnosis of disease or other conditions in humans.Developed to give improved growth rates of Listeria over traditional selective enrichment media, LEE Broth enhances the expression of target antigens for most commercially available immunological test kits/methods whilst maintaining adequate suppression of potential non-target organisms. Selective components are blended into the powder, removing the requirement for supplementation.Compared with more traditional methods, immunological tests, such as ELISA, require relatively high levels of target organisms to achieve a reliable positive result. LEE Broth has been specifically designed to stimulate growth from low numbers to the required high levels within a 24 hour period.For more information visit https://www.neogen.com/solutions/Microbiology/lee-broth
NEOGEN Nutrient Broth, 5 kg
Nutrient Broth is used for the cultivation of a wide variety of microorganisms. Nutrient Broth is not intended for use in the diagnosis of disease or other conditions in humans.In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Broth is the same formulation as Nutrient Agar, only Agar has been omitted.Nutrient Broth is used as a pre-enrichment medium when testing certain foods and dairy products for Salmonella spp. In dried or processed foods, salmonellae may be sub-lethally injured and in low numbers. The presence of other bacteria and food sample components may hinder growth and recovery of Salmonella spp.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-broth
NEOGEN D/E Neutralizing Broth without Tween, 500 g
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth without Tween is not intended for use in the diagnosis of disease or other conditions in humans.D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer.For more information visit https://www.neogen.com/solutions/Microbiology/de-neutralizing-broth-without-tween
NEOGEN Presence Absence Broth, 10 kg
Presence Absence Broth is used for the detection of coliform bacteria in water treatment plants or distribution systems using the presence-absence coliform test in a laboratory setting. Presence Absence Broth is not intended for use in the diagnosis of disease or other conditions in humans. The Presence Absence (PA) test is a presumptive detection for coliforms in water. The test is a simple modification of the multiple-tube procedure. One 100 mL test sample is inoculated into a single culture bottle to obtain qualitative information on the presence or absence of coliforms, through the presence or absence of lactose fermentation. This test is based on the principle that coliforms and other pollution indicator organisms should not be present in a 100 mL water sample.For more information visit https://www.neogen.com/solutions/Microbiology/presence-absense-broth